Pruning-based identification of domain ontologies

We present a novel approach of extracting a domain ontology from large-scale thesauri. Concepts are identified to be relevant for a domain based on their frequent occurrence in domain texts. The approach allows to bootstrap the ontology engineering process from given legacy thesauri and identifies an initial domain ontology that may easily be refined by experts in a later stage. We present a thorough evaluation of the results obtained in building a biosecurity ontology for the UN FAO AOS project

A Total Cost of Ownership Model for Cloud Computing Infrastructure

A holistic cost assessment of cloud computing ar-chitectures is currently hampered by the lack of assessment methods and the absence of a standardized and comprehensive total cost model. This creates uncertainty about cost developments of concrete scenarios and architectural changes. This article proposes a total cost of ownership model for cloud computing, covering the cost of adoption, procurement, migration, operation, usage, and exit. We evaluated our model in multiple application scenarios and against other models. Our model has shown to be substantially more comprehensive and applicable than other available models for cloud computing. Thus, our model can be useful both in practice and in research. We will demonstrate that our model can increase cost transparency and improve decision support

Roadmap for Optical Tweezers 2023

Optical tweezers are tools made of light that enable contactless pushing, trapping, and manipulation of objects ranging from atoms to space light sails. Since the pioneering work by Arthur Ashkin in the 1970s, optical tweezers have evolved into sophisticated instruments and have been employed in a broad range of applications in life sciences, physics, and engineering. These include accurate force and torque measurement at the femtonewton level, microrheology of complex fluids, single micro- and nanoparticle spectroscopy, single-cell analysis, and statistical-physics experiments. This roadmap provides insights into current investigations involving optical forces and optical tweezers from their theoretical foundations to designs and setups. It also offers perspectives for applications to a wide range of research fields, from biophysics to space exploration

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

ABSTRACT Bubo- Implementing OWL in rule-based systems

The Semantic Web is build around a semi-structured data model-RDF- and an explicit conceptualization for such data- so-called ontologies. A standardized language for the specification of the latter has recently be proposed by the W3C. This paper explores the strategies for the implementation of this language in logic programming environments such as Prolog and relational databases. Along these lines we establish the subset of OWL primitives that is compatible for further rule-based extensions paving the way to the upper levels of the Semantic Web layer cake. We also capture a subset, for which query languages can easily be implemented by compiling expressions given in a declarative query language to the query language offered by the system on top of which the implementation is based. 1